实验室紫外线安全防护

实验室紫外线安全防护

实验室中使用的许多紫外线光源都会发出高强度的紫外线，从而导致眼睛和皮肤的疼痛烧伤。本培训演示提供了与紫外线暴露相关的危险以及使用这些来源时应采取的安全预防措施的信息。

上图：实验室常见的紫外线光源警告标志

手持式紫外线灯

手持式紫外线灯可用于研究实验室，用于凝胶电泳和溴化乙锭染色后的核酸可视化或用于其他目的。

手持式紫外灯操作注意事项

•佩戴紫外线防护面罩。

•覆盖所有暴露的皮肤。

•注意可能将紫外线辐射反射到皮肤未受保护部位的反射表面。

实验室人员紫外线防护装备

紫外线防护服：穿全扣实验服、长裤和闭趾鞋。确保所有皮肤都得到保护，包括面部、颈部、手部和手臂。确保你的防护服没有缝隙，尤其是手腕和颈部。

紫外线防护手套：戴上一次性乳胶或丁腈手套，保护手上裸露的皮肤。不要使用乙烯基手套，因为乙烯基手套会传播大量光化紫外线。

眼部/面部防护：始终佩戴全脸防护紫外线防护面罩。为了保护眼睛和面部，请使用印有ANSI Z87.1-1989紫外线认证的聚碳酸酯面罩。注意：如果您在有飞溅或抛射危险的情况下工作，您可能还需要在面罩下佩戴安全眼镜或护目镜。

紫外线防护面罩

只能使用具有紫外线防护等级的聚碳酸酯面罩。面罩应标有术语Z87，以表明面罩符合ANSI标准，至少能提供基本的紫外线防护。阅读面罩提供的规格，以验证面罩是否提供足够的紫外线防护。

紫外线防护面罩可能无法完全保护您。例如，假设你穿着个人防护装备在透照器上工作，但你抬头与附近的人交谈。这会使你的喉咙和下巴暴露在过量的紫外线辐射下。你必须确保没有裸露的皮肤。切勿为了更仔细地观察正在观察的材料而取下紫外线防护面罩。

Ultraviolet Light Safety in the Laboratory

Many of the ultraviolet (UV) sources used in the laboratory emit high intensities of UV light, capable of producing painful eye and skin burns. This training presentation provides information about the hazards associated with UV exposure and the safety precautions to take when working with these sources.

Hand Held UV amps

Handheld UV lamps may be used in research laboratories for visualizing nucleic acids following gel electrophoresis and ethidium bromide staining or for other purposes.

Precautions

• Wear a face shield.

• Cover all exposed skin.

• Be aware of reflective surfaces which can reflect UV radiation to unprotected parts of your skin.

Personnel Protective Equipment

Protective Clothing: Wear a fully buttoned lab coat, long pants and closed toe shoes. Make sure that ALL skin is protected, including face, neck, hands and arms. Make sure there are no gaps in your protective clothing, especially at the wrist and neck areas. 

Gloves: Wear disposable latex or nitrile gloves to protect exposed skin on the hands. Do not use vinyl gloves, which can transmit significant amounts of actinic UV. 

Eye/Face Protection: Always wear a full face shield. To protect the eyes and face, use a polycarbonate face shield stamped with the ANSI Z87.1-1989 UV certification. Note: if you’re working with splash or projectile hazards, you may also need to wear safety glasses or goggles under the face shield.

Face Shields

Only use polycarbonate face shields that are rated for UV protection. The face shield should be marked with the term Z87 to indicate that the shield meets the ANSI standard to provide at least basic UV protection. Read the specs provided with the face shield to verify that the shield provides adequate UV protection.

You may not be totally protected by the face shield. For example, suppose that you are working over a transilluminator, wearing PPE, but you are looking up while talking to someone nearby. This would expose your throat and chin to excessive UV radiation. You must be sure that there is no exposed skin. Never remove the face shield in order to get a closer look at the material being visualized.