紫外灯用于TLC薄层色谱法

紫外灯的应用：薄层色谱法 Thin-layer Chromatography (TLC)

紫外灯波长的选择：根据TLC板类型选择波长，短波(254nm)可用于二氧化硅涂层，长波(365nm)可用于纤维素涂层

WAVELENGTHSI LAMPS USED: Shortwave (254nm) or longwave (365nm) depending upon the type of TLC plate used (Silica coated =254nm, Cellulose coated = 365nm)

薄层色谱法(TLC)是一种微量、快速的层析方法。在生物、医药、化工各个领域都有广泛的应用。色谱法使化学家能够根据其他已知标准测量给定溶剂中未知化合物的溶解度，从而将化合物混合物分离为其成分。薄层色谱 (TLC) 在TLC板上完成，该TLC板由固体支撑物（即玻璃、塑料或铝）组成，并涂有一层薄薄的吸收材料（例如二氧化硅或纤维素）。TLC 板分为“泳道” 当板的一端放入溶剂中时，已知和未知的样品通过毛细作用沿着吸收材料沉积和迁移。相同的化合物将迁移相似的距离并表现出相同的浓度。

Chromatography enables the chemist to separate a mixture of compounds into its constituents by measuring solubilities of unknown compounds in a given solvent against other known standards. Thin-layer Chromatography (TLC) is done on a TLC plate consisting of a solid support, i.e., glass, plastic, or aluminum, coated with a thin layer of absorbent material, such as silica or cellulose.TLC plates are divided into “lanes" where known and unknown samples are deposited and migrate along the absorbent material by capillary action when one end of the plate is placed in solvent. Identical compounds will migrate similar distances and exhibit equal concentrations.

TLC薄层色谱法步骤

已知和未知样品被“点样”到TLC板的泳道上，并用适当的溶剂迁移。通过将板从溶剂中取出并用吹风机从泳道中蒸发溶剂来固定板。溶解的化合物将留在泳道的吸附材料中。然后通过向该板喷洒一种溶液来显影该板，该溶液可诱导存在的化合物的荧光。在析浦紫外灯下照射，如果未知化合物与标准品迁移相同的距离并表现出相同程度的荧光，则认为它们与已知标准品相同。

Known and unknown samples are “spotted” on to lanes of the TLC plate and migrated with appropriate solvent. The plate is fixed by removing it from the solvent and evaporating solvent from the lanes with the hair dryer. The dissolved compounds will be left behind in the adsorbent material of the lanes. The plate is then developed by spraying it with a solution that induces fluorescence of the compounds present and read under ultraviolet light in a fluorescence analysis cabinet. Unknown compounds are considered to be identical to known standards if they migrate the same distance and exhibit the same degree of fluorescence as standards.

PRIMARY ADVANTAGES OF THIS METHOD: Thin-layer chromatography is a simple, versatile, effective and economical test that is relevant to a wide range of separation problems.

析浦科学仪器有限公司生产有各种波段的紫外线灯，有手持式多波段紫外线灯、台式紫外线灯、紫外线观察箱等设备用于TLC薄层色谱分析。